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recombinant human epidermal growth factor  (PromoCell)


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    PromoCell recombinant human epidermal growth factor
    Recombinant Human Epidermal Growth Factor, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+epidermal+growth+factor/ECGS%2C+Endothelial+Cell+Growth+Supplement/pm42003612-131-30-35
    Average 95 stars, based on 153 article reviews
    recombinant human epidermal growth factor - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Inhibition of angiogenesis and tumor progression of MK-0429, an integrin αvβ 3 antagonist, on oral squamous cell carcinoma
    Article Snippet: HUEhT-1 (JCRB1458) cells were purchased from Japanese Collection of Research Bioresources (JCRB) Cell Bank (Osaka, Japan). .. They were cultured at 37 °C in a humidified atmosphere in 5% CO 2 in air and maintained with endothelial cell growth medium supplemented with 0.02 ml/ml fetal calf serum, 5 ng/ml recombinant human epidermal growth factor, 10 ng/ml recombinant human basic fibroblast growth factor, 20 ng/ml insulin-like growth factor, 0.5 ng/ml recombinant human vascular growth factor-165, 1.0 μg/ml ascorbic acid, 22.5 μg/ml heparin, and 0.2 μg/ml hydrocortisone (Promo Cell, Heidelberg, Germany). .. Culture plates and dishes were coated with 0.5 μg/cm 2 vitronectin (A14700, Gibco, Carlsbad, CA, USA) in advance of cell culture.

    Article Title: Genetic transfer of fusion proteins effectively inhibits VCAM-1-mediated cell adhesion and transmigration via inhibition of cytoskeletal anchorage
    Article Snippet: .. HUVECs were obtained from PromoCell (Heidelberg, Germany) and cultured in 0.5% gelatin-coated culture plates filled with ECGM supplemented with basic human recombinant fibroblast growth factor 1.0 ng/ml, endothelial cell growth supplement/heparin 4 μl/ml, FBS 2%, recombinant human epidermal growth factor 0.1 ng/ml, hydrocortisone 1 μg/ml, amphotericin B 50 ng/ml, gentamicin 50 μg/ml; all from PromoCell (Heidelberg, Germany). .. HMEC-1 cells were a gift from H. Eltzschig (University of Tübingen, Germany) and were cultured in MCDB 131 medium supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 0.3 mg/ml L-glutamine, 10% FCS, 2 ng/ml hydrocortisone, 10 ng/ml epidermal growth factor (all from Invitrogen, Carlsbad, CA, USA).

    Article Title: Conserved regulatory logic at accessible and inaccessible chromatin during the acute inflammatory response in mammals
    Article Snippet: .. Primary ECs isolated from aortas of human (two 21 year old Caucasian males, lot# 2139 and 1487; a 15 year old male, lot# 2102; and a 60 year old male, lot# 2366; Cell Application cat# 304-05a), mouse (two biological replicates of C57BL/6 males pooled from multiple mice, Cell Biologics cat# C57-6052, lot# A092913T2MP and B092913T2MP), and cow (two biological replicates, Cell Applications cat# B304-05, lot# 1165 and 1190) were thawed into T75 cell culture flasks and then further grown in T225 flasks at 37 °C and 5% CO 2 in Endothelial Cell Growth Media MV2 (PromoCell) supplemented with 5% fetal calf serum (PromoCell), 5-ng/ml recombinant human epidermal growth factor (PromoCell), 0.5-ng/ml recombinant human vascular endothelial growth factor 165 (PromoCell), 10-ng/ml recombinant human basic fibroblast growth factor (PromoCell), 20-ng/ml long R3 insulin-like growth factor-1 (PromoCell), 1-μg/ml ascorbic acid (PromoCell), and 0.2-μg/ml hydrocortisone (PromoCell). ..

    Article Title: Inhibition of angiogenesis and tumor progression of MK-0429, an integrin αvβ 3 antagonist, on oral squamous cell carcinoma.
    Article Snippet: HUEhT-1 (JCRB1458) cells were purchased from Japanese Collection of Research Bioresources (JCRB) Cell Bank (Osaka, Japan). .. They were cultured at 37 °C in a humidified atmosphere in 5% CO2 in air and maintained with endothelial cell growth medium supplemented with 0.02 ml/ml fetal calf serum, 5 ng/ml recombinant human epidermal growth factor, 10 ng/ml recombinant human basic fibroblast growth factor, 20 ng/ml insulin-like growth factor, 0.5 ng/ml recombinant human vascular growth factor-165, 1.0 μg/ml ascorbic acid, 22.5 μg/ml heparin, and 0.2 μg/ml hydrocortisone (Promo Cell, Heidelberg, Germany). .. Culture plates and dishes were coated with 0.5 μg/cm2 vitronectin (A14700, Gibco, Carlsbad, CA, USA) in advance of cell culture.

    Article Title: Conserved regulatory logic at accessible and inaccessible chromatin during the acute inflammatory response in mammals.
    Article Snippet: .. Primary ECs isolated from aortas of human (two 21 year old Caucasian males, lot# 2139 and 1487; a 15 year old male, lot# 2102; and a 60 year old male, lot# 2366; Cell Application cat# 304-05a), mouse (two biological replicates of C57BL/6 males pooled from multiple mice, Cell Biologics cat# C57-6052, lot# A092913T2MP and B092913T2MP), and cow (two biological replicates, Cell Applications cat# B304-05, lot# 1165 and 1190) were thawed into T75 cell culture flasks and then further grown in T225 flasks at 37 °C and 5% CO2 in Endothelial Cell Growth Media MV2 (PromoCell) supplemented with 5% fetal calf serum (PromoCell), 5-ng/ml recombinant human epidermal growth factor (PromoCell), 0.5-ng/ml recombinant human vascular endothelial growth factor 165 (PromoCell), 10-ng/ml recombinant human basic fibroblast growth factor (PromoCell), 20-ng/ml long R3 insulin-like growth factor-1 (PromoCell), 1-μg/ml ascorbic acid (PromoCell), and 0.2-μg/ml hydrocortisone (PromoCell). ..

    Recombinant:

    Article Title: Inhibition of angiogenesis and tumor progression of MK-0429, an integrin αvβ 3 antagonist, on oral squamous cell carcinoma
    Article Snippet: HUEhT-1 (JCRB1458) cells were purchased from Japanese Collection of Research Bioresources (JCRB) Cell Bank (Osaka, Japan). .. They were cultured at 37 °C in a humidified atmosphere in 5% CO 2 in air and maintained with endothelial cell growth medium supplemented with 0.02 ml/ml fetal calf serum, 5 ng/ml recombinant human epidermal growth factor, 10 ng/ml recombinant human basic fibroblast growth factor, 20 ng/ml insulin-like growth factor, 0.5 ng/ml recombinant human vascular growth factor-165, 1.0 μg/ml ascorbic acid, 22.5 μg/ml heparin, and 0.2 μg/ml hydrocortisone (Promo Cell, Heidelberg, Germany). .. Culture plates and dishes were coated with 0.5 μg/cm 2 vitronectin (A14700, Gibco, Carlsbad, CA, USA) in advance of cell culture.

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria.
    Article Snippet: HCMECs from PromoCell (C-12285) were used up to passage 8. .. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90 μg/mL heparin (PromoCell C-30120), 10 ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1 μg/mL hydrocortisone (PromoCell C-31061). .. HCMEC experiments were performed in endothelial cell growth media MV (PromoCell) with no supplements.

    Article Title: Genetic transfer of fusion proteins effectively inhibits VCAM-1-mediated cell adhesion and transmigration via inhibition of cytoskeletal anchorage
    Article Snippet: .. HUVECs were obtained from PromoCell (Heidelberg, Germany) and cultured in 0.5% gelatin-coated culture plates filled with ECGM supplemented with basic human recombinant fibroblast growth factor 1.0 ng/ml, endothelial cell growth supplement/heparin 4 μl/ml, FBS 2%, recombinant human epidermal growth factor 0.1 ng/ml, hydrocortisone 1 μg/ml, amphotericin B 50 ng/ml, gentamicin 50 μg/ml; all from PromoCell (Heidelberg, Germany). .. HMEC-1 cells were a gift from H. Eltzschig (University of Tübingen, Germany) and were cultured in MCDB 131 medium supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 0.3 mg/ml L-glutamine, 10% FCS, 2 ng/ml hydrocortisone, 10 ng/ml epidermal growth factor (all from Invitrogen, Carlsbad, CA, USA).

    Article Title: Conserved regulatory logic at accessible and inaccessible chromatin during the acute inflammatory response in mammals
    Article Snippet: .. Primary ECs isolated from aortas of human (two 21 year old Caucasian males, lot# 2139 and 1487; a 15 year old male, lot# 2102; and a 60 year old male, lot# 2366; Cell Application cat# 304-05a), mouse (two biological replicates of C57BL/6 males pooled from multiple mice, Cell Biologics cat# C57-6052, lot# A092913T2MP and B092913T2MP), and cow (two biological replicates, Cell Applications cat# B304-05, lot# 1165 and 1190) were thawed into T75 cell culture flasks and then further grown in T225 flasks at 37 °C and 5% CO 2 in Endothelial Cell Growth Media MV2 (PromoCell) supplemented with 5% fetal calf serum (PromoCell), 5-ng/ml recombinant human epidermal growth factor (PromoCell), 0.5-ng/ml recombinant human vascular endothelial growth factor 165 (PromoCell), 10-ng/ml recombinant human basic fibroblast growth factor (PromoCell), 20-ng/ml long R3 insulin-like growth factor-1 (PromoCell), 1-μg/ml ascorbic acid (PromoCell), and 0.2-μg/ml hydrocortisone (PromoCell). ..

    Article Title: Plasmodium falciparum hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an in vitro model of cerebral malaria
    Article Snippet: HCMECs from PromoCell (C-12285) were used up to passage 8. .. HCMECs were maintained in Endothelial Cell Growth Media MV (PromoCell C-22120) supplemented with 5% FCS (PromoCell C-37310), 0.4% endothelial cell growth supplement with 90μg/mL heparin (PromoCell C-30120), 10ng/mL recombinant human epidermal growth factor (PromoCell C-30226), and 1μg/mL hydrocortisone (PromoCell C-31061). .. HCMEC experiments were performed in endothelial cell growth media MV (PromoCell) with no supplements.

    Article Title: Air-Pressure-Supported Application of Cultured Human Keratinocytes in a Fibrin Sealant Suspension as a Potential Clinical Tool for Large-Scale Wounds.
    Article Snippet: .. 2022, 11, 5032 3 of 10 nant human transferrin-5, 0.33 μg/mL hydrocortisone-165, 0.39 μg/mL epinephrine-195, 0.004 mL/mL bovine pituitary extract, 0.125 ng/mL recombinant human epidermal growth factor, 5 μL/mL recombinant human insulin, 0.33 μg/mL hydrocortisone-165, 0.39 μg/mL epinephrine-195 (all from PromoCell GmbH, Heidelberg, Germany), and 1% penicillin– streptomycin (Sigma-Aldrich Corporation, St. Louis, MO, USA). .. We incubated the cells at 37 ◦C, 5% CO2 in cell culture flasks (T75) coated with 3.0 mg/cm2 rat tail collagen type I (Sigma-Aldrich Corporation, St. Louis, MO, USA).

    Article Title: Inhibition of angiogenesis and tumor progression of MK-0429, an integrin αvβ 3 antagonist, on oral squamous cell carcinoma.
    Article Snippet: HUEhT-1 (JCRB1458) cells were purchased from Japanese Collection of Research Bioresources (JCRB) Cell Bank (Osaka, Japan). .. They were cultured at 37 °C in a humidified atmosphere in 5% CO2 in air and maintained with endothelial cell growth medium supplemented with 0.02 ml/ml fetal calf serum, 5 ng/ml recombinant human epidermal growth factor, 10 ng/ml recombinant human basic fibroblast growth factor, 20 ng/ml insulin-like growth factor, 0.5 ng/ml recombinant human vascular growth factor-165, 1.0 μg/ml ascorbic acid, 22.5 μg/ml heparin, and 0.2 μg/ml hydrocortisone (Promo Cell, Heidelberg, Germany). .. Culture plates and dishes were coated with 0.5 μg/cm2 vitronectin (A14700, Gibco, Carlsbad, CA, USA) in advance of cell culture.

    Article Title: Conserved regulatory logic at accessible and inaccessible chromatin during the acute inflammatory response in mammals.
    Article Snippet: .. Primary ECs isolated from aortas of human (two 21 year old Caucasian males, lot# 2139 and 1487; a 15 year old male, lot# 2102; and a 60 year old male, lot# 2366; Cell Application cat# 304-05a), mouse (two biological replicates of C57BL/6 males pooled from multiple mice, Cell Biologics cat# C57-6052, lot# A092913T2MP and B092913T2MP), and cow (two biological replicates, Cell Applications cat# B304-05, lot# 1165 and 1190) were thawed into T75 cell culture flasks and then further grown in T225 flasks at 37 °C and 5% CO2 in Endothelial Cell Growth Media MV2 (PromoCell) supplemented with 5% fetal calf serum (PromoCell), 5-ng/ml recombinant human epidermal growth factor (PromoCell), 0.5-ng/ml recombinant human vascular endothelial growth factor 165 (PromoCell), 10-ng/ml recombinant human basic fibroblast growth factor (PromoCell), 20-ng/ml long R3 insulin-like growth factor-1 (PromoCell), 1-μg/ml ascorbic acid (PromoCell), and 0.2-μg/ml hydrocortisone (PromoCell). ..

    Isolation:

    Article Title: Conserved regulatory logic at accessible and inaccessible chromatin during the acute inflammatory response in mammals
    Article Snippet: .. Primary ECs isolated from aortas of human (two 21 year old Caucasian males, lot# 2139 and 1487; a 15 year old male, lot# 2102; and a 60 year old male, lot# 2366; Cell Application cat# 304-05a), mouse (two biological replicates of C57BL/6 males pooled from multiple mice, Cell Biologics cat# C57-6052, lot# A092913T2MP and B092913T2MP), and cow (two biological replicates, Cell Applications cat# B304-05, lot# 1165 and 1190) were thawed into T75 cell culture flasks and then further grown in T225 flasks at 37 °C and 5% CO 2 in Endothelial Cell Growth Media MV2 (PromoCell) supplemented with 5% fetal calf serum (PromoCell), 5-ng/ml recombinant human epidermal growth factor (PromoCell), 0.5-ng/ml recombinant human vascular endothelial growth factor 165 (PromoCell), 10-ng/ml recombinant human basic fibroblast growth factor (PromoCell), 20-ng/ml long R3 insulin-like growth factor-1 (PromoCell), 1-μg/ml ascorbic acid (PromoCell), and 0.2-μg/ml hydrocortisone (PromoCell). ..

    Article Title: Conserved regulatory logic at accessible and inaccessible chromatin during the acute inflammatory response in mammals.
    Article Snippet: .. Primary ECs isolated from aortas of human (two 21 year old Caucasian males, lot# 2139 and 1487; a 15 year old male, lot# 2102; and a 60 year old male, lot# 2366; Cell Application cat# 304-05a), mouse (two biological replicates of C57BL/6 males pooled from multiple mice, Cell Biologics cat# C57-6052, lot# A092913T2MP and B092913T2MP), and cow (two biological replicates, Cell Applications cat# B304-05, lot# 1165 and 1190) were thawed into T75 cell culture flasks and then further grown in T225 flasks at 37 °C and 5% CO2 in Endothelial Cell Growth Media MV2 (PromoCell) supplemented with 5% fetal calf serum (PromoCell), 5-ng/ml recombinant human epidermal growth factor (PromoCell), 0.5-ng/ml recombinant human vascular endothelial growth factor 165 (PromoCell), 10-ng/ml recombinant human basic fibroblast growth factor (PromoCell), 20-ng/ml long R3 insulin-like growth factor-1 (PromoCell), 1-μg/ml ascorbic acid (PromoCell), and 0.2-μg/ml hydrocortisone (PromoCell). ..



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